A CEP1 peptide receptor regulates the cluster root morphology in lupinus albus adapted to phosphorus deficiency
Interdisziplinäres Projekt
- Beschreibung
White lupin (Lupinus albus L.) is known for its extreme tolerance to low phosphorus availability and unusual root architecture, it develops short, densely clustered lateral roots (cluster roots) in response to Pi deficiency. Nutrient mobilization mediated by CRs has been characterized most comprehensively using white lupin as a model plant, this special morphology of cluster roots increased root surface area combined with large amounts of root exudates such as citrate, malate as well as flavonoids for P mobilization and absorbtion.
The CEP (C-TERMINALLY ENCODED PEPTIDE) gene family, encoding small secreted peptides that exhibit a conserved 15-amino acid peptide domain at or near the C-terminus, was discovered using an in silico approach. CEP genes were reported to be widespread among seeds plants with true branching roots or vasculature, and the expression patterns are regulated by environmental cues. In dicot plants, one member of the CEP gene family has been shown to arrest root growth, which negatively regulate lateral and primary root development and positively regulate nodulation.
Based on our former results, we found overexpression of CEP1 gene and exogenous addition of CEP1 peptide in Pi-deficiency white lupin arrested cluster roots formation, decreased the rootlets and root hair length. The results of transient expression of CEP1 gene in Nicotiana tabacum leaves indicated that CEP1 gene localized in apoplast, which might undergo the proteolysis and translational modification in white lupin cluster root formation.
Small signaling peptides and their receptors play essential roles in plant growth and development, one peptide can bind or signal through multiple receptors and one receptor can recognize several peptides, therefore, an enormous number of possible peptide ligand-receptor kinase pairs which regulate the plant growth and development. Evidently, a CEP1 peptide ligand-receptor identification is crucial to fully understand receptor kinase-mediated signaling pathways involved in modulation of cluster roots morphology in white lupin.
- Beschreibung des interdisziplinären Teils des Projekts
- This project relates to two disciplines. 1) Molecular biology: in this project, the up to date genome editing technology-CRISPR/Cas9 will be employed to generate the CEP1 receptor loss-of-function mutant white lupin plants; CRISPR/Cas9 constructs creation including the protospacer designing, DNA ligation, DNA transformation and purification; to deliver the CRISPR/Cas9 constructs in to plants, the Agrobacterium mediated plant transformation will be used. 2) Bioinformatics: during the CRISPR/Cas9 constructs creation, we need to send our constructs sequencing for confirmation, afterwards, sequence alignments will be carried out with bioinformatics program.
- Projektzeitraum
- Wintersemester 2017/18 und Sommersemester 2018
- Bewerbungszeitraum
- 16.10. bis 02.11.2017
- Durchführung
- nach Absprache
- Studienfach
- Agrarbiologie
- Betreuende
- Yaping Zhou
- Institut
- Institut für Kulturpflanzenwissenschaften (340) (Molecular Biology)
- Sprache
- englisch
- Teilnehmendenanzahl
- min. 1, max. 2
- Arbeitsaufwand
-
ca. 180 Stunden pro Teilnehmende:r
| 6
ECTS-Punkte
Arbeitsaufwand (Stunden und ggf. ECTS) sind ungefähre Angaben. Die tatsächlich vergebenen ECTS-Punkte ergeben sich aus der tatsächlich geleisteten Arbeit.
- Für dieses Projekt ist kein Motivationsschreiben des Studierenden erforderlich
- Projektart
- experimentell
- Lernziele
-
Die Teilnehmende lernen in diesem Projekt:
After finish this project, the students will get the skill as follows:
1) biological experiments design
2) DNA clone, transformation and purification
3) Agrobacterium mediated plant transformation
- Anmerkungen für Studierende