Calcium-Nitrate Crosstalk
- Beschreibung
The calcium concentrations in the cytosol are tightly controlled to avoid toxicity and moreover, the release of specific amounts of calcium from its stores orchestrates a signalling cascade. The dynamics of calcium signalling and its specificity in terms of its amplitude, duration, pattern are slowly unfolding. Reporter lines with aequorin, GCaMPs, etc. have been employed in decoding the specificity of calcium signalling, its role and pathways. The signals released from these reporter lines can be used to decipher calcium’s role in a specific pathway by applying a ‘stressor’. Calcium signalling also has a role in the nitrate pathway and with the help of reporter lines we would like to study how calcium plays a role in this pathway.
During the course of this project, we would like to explore the crosstalk between calcium and nitrate using the aequorin reporter line (WT-AEQ) and by checking the transcripts of hydroponically grown plants subjected to various nitrate treatments.
- Projektzeitraum
- Wintersemester 2025/2026 und Sommersemester 2026
- Bewerbungszeitraum
- 13. bis 27.10.2025
- Durchführung
- nach Absprache
- Studienfach
- offen für alle Studienfächer
- Betreuende
- Rebecca John, Dr. Tatsiana Straub
- Institut
- Institut für Biologie (190) (Plant Systems Biology)
- Sprache
- englisch
- Teilnehmendenanzahl
- min. 1, max. 1
- Arbeitsaufwand
-
ca. 120 Stunden pro Teilnehmende:r
| 4
ECTS-Punkte
Arbeitsaufwand (Stunden und ggf. ECTS) sind ungefähre Angaben. Die tatsächlich vergebenen ECTS-Punkte ergeben sich aus der tatsächlich geleisteten Arbeit.
- Für dieses Projekt ist kein Motivationsschreiben des Studierenden erforderlich
- Projektart
- experimentell
- Lernziele
-
Die Teilnehmende lernen in diesem Projekt:
- Measure and quantify luminescence signals from an aequorin line (WT-AEQ)
- Establish a method for the growth conditions of the reporter lines
- Hydroponic cultivation of Arabidopsis plants
- Check the transcriptional response using qPCR
- Plan and set-up and experiment
- Anmerkungen für Studierende