Intra-specific variation in protease inhibitors their relation to herbivore resistance in natural populations of Solanum dulcamara
- Beschreibung
S. dulcamara commonly referred to as Bittersweet Nightshades, are an emerging model system for studying plant-insect interactions. S. dulcamara plants are phenotypically plastic which are reflected by numerous inducible traits.
In this project, we would be working with specialist and generalist leaf chewing beetles lepidopterans. The goal is to explore, whether S. dulcamara can differentially respond to different chewing herbivores. For this, clones of selected chemically distinct plant genotypes are exposed to different insects. Imprints of different herbivores on the leaf chemistry are assessed for defence-related parameters such as polyphenols, protease inhibitors (PI) and/or the induction of signalling pathways. Polyphenolics and phytohormones can be analysed by liquid chromatography, defense gene expression by real time PCR and PIs with biochemical assay.
- Projektzeitraum
- Wintersemester 2023/24 und Sommersemester 2024
- Bewerbungszeitraum
- 16.10. bis 06.11.2023
- Durchführung
- nach Absprache
- Studienfach
-
Agrarbiologie
Agrarwissenschaften
Biologie
Lebensmittelchemie
Nachwachsende Rohstoffe und Bioenergie - Betreuende
- Kruthika Sen Aragam, Prof.Dr. Anke Steppuhn
- Institut
- Institut für Biologie (190) (Molecular Botany 190a)
- Sprache
- englisch
- Teilnehmendenanzahl
- min. 1, max. 2
- Arbeitsaufwand
-
ca. 120 Stunden pro Teilnehmende:r
| 4
ECTS-Punkte
Arbeitsaufwand (Stunden und ggf. ECTS) sind ungefähre Angaben. Die tatsächlich vergebenen ECTS-Punkte ergeben sich aus der tatsächlich geleisteten Arbeit.
- Für dieses Projekt ist kein Motivationsschreiben des Studierenden erforderlich
- Projektart
- experimentell
- Lernziele
-
Die Teilnehmende lernen in diesem Projekt:
Insights in ecology and evolution of plant-insect interaction
lern the application of diffrent scientific methods: Chemical analytics (HPLC-DAD, Protein inhibitor activity assay, protein quantification, handling of and experiments with insects and plants, Data analysis and statistical methods
- Anmerkungen für Studierende
I have attached (PDF) a general project theme I am working on, if you are interested, you could have a quick glance.
If you have more questions please contact me via kruthika.senaragam@uni-hohenheim.de
- Schlagworte
- plant defense responses, insects, chemical-analytics